In-vitro Study on Α-amylase Inhibitory Activity and Phytochemical Screening of Mint Leaves and Annona Squamosa Leaves Having Anti-diabetic Properties

α-amylase inhibitors are used in the treatment of type II diabetes mellitus. This study aims to identify the α-amylase inhibitors from two medicinal plants Mentha piperita and Annona squamosa. Primarily, the extract of both leaves was prepared using methanol and water and perform the screening of phytochemicals constituents present in the extract. Aqueous and methanol extract of mentha leaves contains like glycosides, steroids, alkaloids, flavonoids, tannins, saponins and A. squamosa leaves extract contains flavonoids, triterpenoids, tannins, and quinines. Furthermore, the α-amylase inhibitors of both plant’s aqueous and methanol extract was analyzed by spectroscopic method. The results indicated both plants whose methanolic extracts exhibited highest α-amylase inhibitory activity were M. piperita and A. squamosa with IC50 of 30.84±1.03 and 47.78±2.45 μg/ml, respectively. This finding could be used to support the use of these plants for treatment of diabetes and also it is an interesting phyto-chemicals to be developed as a new drug.